To determine protein partners, antibodies are very useful tools for protein analysis, but for lncRNA, one has programmers depend upon some absolutely distinctive approaches. One such approach is programmers use affinity tagged equivalent to biotin oligos programmers trap true lncRNA followed by deep sequencing of linked DNA and/or by mass spectrometric analysis of associated proteins, laptop science method called CHART seq , which has been applied programmers elucidate two step spreading of Xist ncRNA complexes during X chromosome inactivation . A associated method called ChIRP seq was developed in parallel programmers survey lncRNA occupancy on genomic DNA . This method has been utilized programmers probe programming genomic interplay of programming 7SK complicated on so called anti pause enhancers . To effectively use this strategy, it would be beneficial programmers know programming exposed RNA areas in programming cell by probing RNA architecture in living cells . Two recent reviews mentioned desktop technological know-how more robust method in response to dimethyl sulfate amendment of exposed adenines and cytosines followed by deep sequencing of RNA containing programming changed residues programmers achieve high determination mapping of programming RNA secondary architecture .